| A Star is Born (Bronze) | Complete any level with a 5 star rating |
| Bridge Commander (Bronze) | Beat the top score on the 'Atrium' Challenge |
| Collector's Delight (Bronze) | Collect all weapons, text logs and audio logs on Chapter 1: 'Worlds Apart' |
| Colony Full Of Crazy People (Bronze) | Beat the top score on the 'Medical Bay' Challenge |
| Engineer By Trade (Bronze) | Beat the top score on the 'Maintenance Bay' Challenge |
| Got A Medic (Bronze) | Beat the top score on the 'Life Support Room' Challenge |
| Gun Dealer (Silver) | Collect every weapon on one profile |
| Herbal Remedies (Bronze) | Beat the top score on the 'East Grow Chamber' Challenge |
| Isaac 2.0 (Gold) | Complete the game on 'Impossible' |
| It Needs Our Help (Bronze) | Complete Chapter 1: 'Worlds Apart' |
| Laugh It Up Partner (Bronze) | Beat the top score on the 'Mess Hall' Challenge |
| Make Us Whole (Bronze) | Collect all weapons, text logs and audio logs on Chapter 9: 'Escaping the Ishimura' |
| Man Up Sweetheart (Platinum) | Unlock all trophies |
| Mega Star (Gold) | Earn 50 stars on one profile |
| Necro Slayer (Silver) | Complete the game on 'Expert' |
| Need A Towel (Bronze) | Beat the top score on the 'Water Tunnels' Challenge |
| No Cop Duty (Bronze) | Beat the top score on the 'Central Plaza' Challenge |
| No Stone Unturned (Bronze) | Collect all weapons, text logs and audio logs on Chapter 3: 'Return to the Megavents' |
| Overtime Sheet (Bronze) | Beat the top score on the 'Extraction Site' Challenge |
| P-Sec Officer (Silver) | Complete the game on 'Hard' |
| Ready For Takeoff (Bronze) | Beat the top score on the 'Shuttle Bay 47' Challenge |
| Rising Star (Bronze) | Earn 10 stars on one profile |
| Scavenger (Bronze) | Collect all weapons, text logs and audio logs on Chapter 5: 'Emergency Care' |
| Skill - Accuracy (Bronze) | Complete any level with at least 80% Weapon Accuracy |
| Skill - Reload (Bronze) | Complete any level with at least 80% Perfect Reloads |
| Skill - Stasis (Bronze) | Complete any level with at least 80% Stasis Accuracy |
| Super Star (Silver) | Earn 30 stars on one profile |
| Survivor (Silver) | Complete the game on 'Normal' |
| That's Quite A Mouthful (Bronze) | Complete Chapter 2: 'Another Day at the Office' |
| Too Much Firepower? (Bronze) | Open the secret weapons store in P-Sec |
| Treasure Hunter (Bronze) | Collect all weapons, text logs and audio logs on Chapter 8: 'In the Hearts of Men' |
| Weapon Nut (Gold) | Max out all upgrades for every weapon on one profile |
| You Need Glasses Partner? (Bronze) | Survive the shuttle flight with less than 10% of shields intact |
